Genotype-free demultiplexing of pooled single-cell RNA-seq

Genotype-free demultiplexing of pooled single-cell RNA-seq

Abstract A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequencing (scRNA-seq) experiment which either require hashtag barcodes or sample genotypes prior to pooling. We introduce scSplit which utilizes genetic differences inferred from scRNA-seq data alon...

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Bibliographic Details
Main Authors: Jun Xu, Caitlin Falconer, Quan Nguyen, Joanna Crawford, Brett D. McKinnon, Sally Mortlock, Anne Senabouth, Stacey Andersen, Han Sheng Chiu, Longda Jiang, Nathan J. Palpant, Jian Yang, Michael D. Mueller, Alex W. Hewitt, Alice Pébay, Grant W. Montgomery, Joseph E. Powell, Lachlan J.M Coin
Format: Article
Language:English
Published: BMC 2019-12-01
Series:Genome Biology
Subjects:
scSplit
scRNA-seq
Demultiplexing
Machine learning
Unsupervised
Hidden Markov Model
Online Access:https://doi.org/10.1186/s13059-019-1852-7

Internet

https://doi.org/10.1186/s13059-019-1852-7

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