Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli

Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli

The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, an...

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Bibliographic Details
Main Authors: Davoud Koolivand, Nemat Sokhandan Bashir, Seyed Aliakbar Behjatnia, Raziallah Jafari Joozani
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2016-10-01
Series:The Plant Pathology Journal
Subjects:
antibody
ELISA
expression
Grapevine fanleaf virus
recombinant
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051564

Internet

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051564

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