Next-generation sequencing of human mitochondrial reference genomes uncovers high heteroplasmy frequency.
We describe methods for rapid sequencing of the entire human mitochondrial genome (mtgenome), which involve long-range PCR for specific amplification of the mtgenome, pyrosequencing, quantitative mapping of sequence reads to identify sequence variants and heteroplasmy, as well as de novo sequence as...
Main Authors: | , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
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Series: | PLoS Computational Biology |
Online Access: | http://europepmc.org/articles/PMC3486893?pdf=render |