A vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells

Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis.  Retinoic acid was used to dif...

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Bibliographic Details
Main Authors: Reza Ebrahimzadeh-Vesal, Mohammad Ali hokrgozar, Karim Nayernia, Ladan Teimoori-Toolabi, Mohammad Miryounesi, Seyedmehdi Nourashrafeddin, Najmeh Ranji, Mohammad Hosein Modarressi
Format: Article
Language:English
Published: Mashhad University of Medical Sciences 2014-08-01
Series:Iranian Journal of Basic Medical Sciences
Subjects:
Online Access:http://ijbms.mums.ac.ir/pdf_3190_ec4a8784d687d1bd9412eb77003aea81.html