Immune Blot Analysis on Expression of the Mammalian Target of Rapamycin in Goat Fetal Fibroblasts with Recombinant Polyclonal Antibody

Immune Blot Analysis on Expression of the Mammalian Target of Rapamycin in Goat Fetal Fibroblasts with Recombinant Polyclonal Antibody

To detect the mammalian target of rapamycin (mTOR) expressed in Cashmere goat fetal fibroblasts (GFb), mTOR gene was cloned from Inner Mongolia Cashmere goat (Capra hircus) and expressed in Escherichia coli followed by immunizing mice with the purified recombinant protein as an immunogen to produce...

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Bibliographic Details
Main Authors: Yan LIANG, Shu-yu LI, Xiao-jing WANG, Man-lin WU, Jiao-fu YANG, Jie LI, Xi-yan HAO, Hui-fang HAO, Yin-ma BA, Xian-wei CHEN, Zhi-gang WANG
Format: Article
Language:English
Published: Elsevier 2012-06-01
Series:Journal of Integrative Agriculture
Subjects:
prokaryotic expression
recombinant antibody
goat
mTOR
CCI-779
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311912600926

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http://www.sciencedirect.com/science/article/pii/S2095311912600926

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