Development and validation of probe-based multiplex real-time PCR assays for the rapid and accurate detection of freshwater fish species.

Reliable species identification methods are important for industrial environmental monitoring programs. Probe based real-time quantitative polymerase chain reaction (qPCR) provides an accurate, cost-effective and high-throughput method for species identification. Here we present the development and...

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Bibliographic Details
Main Authors: Emily N Hulley, Sujeenthar Tharmalingam, Andrew Zarnke, Douglas R Boreham
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0210165