DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome

DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome

The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence of ATPγS and a DNA duplex bearing a 3′ single-stran...

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Bibliographic Details
Main Authors: Alessandro Costa, Ludovic Renault, Paolo Swuec, Tatjana Petojevic, James J Pesavento, Ivar Ilves, Kirsty MacLellan-Gibson, Roland A Fleck, Michael R Botchan, James M Berger
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-08-01
Series:eLife
Subjects:
DNA replication
Mcm2-7
helicase
motor protein
replication fork
AAA+ ATPase
Online Access:https://elifesciences.org/articles/03273

Internet

https://elifesciences.org/articles/03273

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