CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation
Abstract Background The reported antitumor activity of the BET family bromodomain inhibitors has prompted the development of inhibitors against other bromodomains. However, the human genome encodes more than 60 different bromodomains and most of them remain unexplored. Results We report that the bro...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2018-06-01
|
Series: | Epigenetics & Chromatin |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13072-018-0197-x |
id |
doaj-1cadd9631f0b4b4fbc0049e7261406dc |
---|---|
record_format |
Article |
spelling |
doaj-1cadd9631f0b4b4fbc0049e7261406dc2020-11-25T01:33:31ZengBMCEpigenetics & Chromatin1756-89352018-06-0111111510.1186/s13072-018-0197-xCREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferationVeronica Garcia-Carpizo0Sergio Ruiz-Llorente1Jacinto Sarmentero2Osvaldo Graña-Castro3David G. Pisano4Maria J. Barrero5CNIO-Lilly Epigenetics Laboratory, Spanish National Cancer Research Center (CNIO)CNIO-Lilly Epigenetics Laboratory, Spanish National Cancer Research Center (CNIO)CNIO-Lilly Epigenetics Laboratory, Spanish National Cancer Research Center (CNIO)Bioinformatics Unit, Spanish National Cancer Research Center (CNIO)Bioinformatics Unit, Spanish National Cancer Research Center (CNIO)CNIO-Lilly Epigenetics Laboratory, Spanish National Cancer Research Center (CNIO)Abstract Background The reported antitumor activity of the BET family bromodomain inhibitors has prompted the development of inhibitors against other bromodomains. However, the human genome encodes more than 60 different bromodomains and most of them remain unexplored. Results We report that the bromodomains of the histone acetyltransferases CREBBP/EP300 are critical to sustain the proliferation of human leukemia and lymphoma cell lines. EP300 is very abundant at super-enhancers in K562 and is coincident with sites of GATA1 and MYC occupancy. In accordance, CREBBP/EP300 bromodomain inhibitors interfere with GATA1- and MYC-driven transcription, causing the accumulation of cells in the G0/G1 phase of the cell cycle. The CREBBP/CBP30 bromodomain inhibitor CBP30 displaces CREBBP and EP300 from GATA1 and MYC binding sites at enhancers, resulting in a decrease in the levels of histone acetylation at these regulatory regions and consequently reduced gene expression of critical genes controlled by these transcription factors. Conclusions Our data shows that inhibition of CREBBP/EP300 bromodomains can interfere with oncogene-driven transcriptional programs in cancer cells and consequently hold therapeutic potential.http://link.springer.com/article/10.1186/s13072-018-0197-xEpigeneticsBromodomainsProliferationCancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Veronica Garcia-Carpizo Sergio Ruiz-Llorente Jacinto Sarmentero Osvaldo Graña-Castro David G. Pisano Maria J. Barrero |
spellingShingle |
Veronica Garcia-Carpizo Sergio Ruiz-Llorente Jacinto Sarmentero Osvaldo Graña-Castro David G. Pisano Maria J. Barrero CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation Epigenetics & Chromatin Epigenetics Bromodomains Proliferation Cancer |
author_facet |
Veronica Garcia-Carpizo Sergio Ruiz-Llorente Jacinto Sarmentero Osvaldo Graña-Castro David G. Pisano Maria J. Barrero |
author_sort |
Veronica Garcia-Carpizo |
title |
CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation |
title_short |
CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation |
title_full |
CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation |
title_fullStr |
CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation |
title_full_unstemmed |
CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation |
title_sort |
crebbp/ep300 bromodomains are critical to sustain the gata1/myc regulatory axis in proliferation |
publisher |
BMC |
series |
Epigenetics & Chromatin |
issn |
1756-8935 |
publishDate |
2018-06-01 |
description |
Abstract Background The reported antitumor activity of the BET family bromodomain inhibitors has prompted the development of inhibitors against other bromodomains. However, the human genome encodes more than 60 different bromodomains and most of them remain unexplored. Results We report that the bromodomains of the histone acetyltransferases CREBBP/EP300 are critical to sustain the proliferation of human leukemia and lymphoma cell lines. EP300 is very abundant at super-enhancers in K562 and is coincident with sites of GATA1 and MYC occupancy. In accordance, CREBBP/EP300 bromodomain inhibitors interfere with GATA1- and MYC-driven transcription, causing the accumulation of cells in the G0/G1 phase of the cell cycle. The CREBBP/CBP30 bromodomain inhibitor CBP30 displaces CREBBP and EP300 from GATA1 and MYC binding sites at enhancers, resulting in a decrease in the levels of histone acetylation at these regulatory regions and consequently reduced gene expression of critical genes controlled by these transcription factors. Conclusions Our data shows that inhibition of CREBBP/EP300 bromodomains can interfere with oncogene-driven transcriptional programs in cancer cells and consequently hold therapeutic potential. |
topic |
Epigenetics Bromodomains Proliferation Cancer |
url |
http://link.springer.com/article/10.1186/s13072-018-0197-x |
work_keys_str_mv |
AT veronicagarciacarpizo crebbpep300bromodomainsarecriticaltosustainthegata1mycregulatoryaxisinproliferation AT sergioruizllorente crebbpep300bromodomainsarecriticaltosustainthegata1mycregulatoryaxisinproliferation AT jacintosarmentero crebbpep300bromodomainsarecriticaltosustainthegata1mycregulatoryaxisinproliferation AT osvaldogranacastro crebbpep300bromodomainsarecriticaltosustainthegata1mycregulatoryaxisinproliferation AT davidgpisano crebbpep300bromodomainsarecriticaltosustainthegata1mycregulatoryaxisinproliferation AT mariajbarrero crebbpep300bromodomainsarecriticaltosustainthegata1mycregulatoryaxisinproliferation |
_version_ |
1725076617117564928 |