A mutant Brassica napus (canola) population for the identification of new genetic diversity via TILLING and next generation sequencing.

We have generated a Brassica napus (canola) population of 3,158 EMS-mutagenised lines and used TILLING to demonstrate that the population has a high enough mutation density that it will be useful for identification of mutations in genes of interest in this important crop species. TILLING is a revers...

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Bibliographic Details
Main Authors: Erin J Gilchrist, Christine H D Sidebottom, Chu Shin Koh, Tanya Macinnes, Andrew G Sharpe, George W Haughn
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3869819?pdf=render