Periplasmic synthesis and purification of the human prolactin antagonist Δ1-11-G129R-hPRL

Periplasmic synthesis and purification of the human prolactin antagonist Δ1-11-G129R-hPRL

Abstract The human prolactin antagonist Δ1-11-G129R-hPRL is a 21.9 kDa recombinant protein with 188 amino acids that downregulates the proliferation of a variety of cells expressing prolactin receptors. Periplasmic expression of recombinant proteins in E. coli has been considered an option for obtai...

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Bibliographic Details
Main Authors: Miriam F. Suzuki, Larissa A. Almeida, Stephanie A. Pomin, Felipe D. Silva, Renan P. Freire, João E. Oliveira, Regina Affonso, Carlos R. J. Soares, Paolo Bartolini
Format: Article
Language:English
Published: SpringerOpen 2021-04-01
Series:AMB Express
Subjects:
Prolactin antagonist
Periplasmic expression
DsbA signal peptide
Antagonistic properties
Online Access:https://doi.org/10.1186/s13568-021-01209-5

Internet

https://doi.org/10.1186/s13568-021-01209-5

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