Baboon Envelope Pseudotyped “Nanoblades” Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34+ Cells and Knock-in of AAV6-Encoded Donor DNA in CD34+ Cells

Baboon Envelope Pseudotyped “Nanoblades” Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34+ Cells and Knock-in of AAV6-Encoded Donor DNA in CD34+ Cells

Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into human blood cells can be challenging. Here, we have utilized “nanoblades,” a new technology that delivers a genomic cleaving agent into cells. These are...

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Bibliographic Details
Main Authors: Alejandra Gutierrez-Guerrero, Maria Jimena Abrey Recalde, Philippe E. Mangeot, Caroline Costa, Ornellie Bernadin, Séverine Périan, Floriane Fusil, Gisèle Froment, Adriana Martinez-Turtos, Adrien Krug, Francisco Martin, Karim Benabdellah, Emiliano P. Ricci, Simone Giovannozzi, Rik Gijsbers, Eduard Ayuso, François-Loïc Cosset, Els Verhoeyen
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-02-01
Series:Frontiers in Genome Editing
Subjects:
hematopoietic stem cells
T cell
B cell
gene editing
CRISPR/Cas9
nanoblade
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.604371/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2021.604371/full

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