Agrobacterium tumefaciens – Mediated transformation of Woodfordia fruticosa (L.) Kurz

Agrobacterium tumefaciens – Mediated transformation of Woodfordia fruticosa (L.) Kurz

In the present study, a protocol for Agrobacterium tumefaciens-mediated transformation has been optimized for Woodfordia fruticosa (L.) Kurz. Precultured axenic leaf segments were co-cultivated with A. tumefaciens strain LBA4404 harboring the binary plasmid pCAMBIA1301 with β-glucuronidase (uidA) co...

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Bibliographic Details
Main Authors: Mallesham Bulle, Deepa Rathakatla, Raghuvardhan Lakkam, Venugopal Rao Kokkirala, Mahender Aileni, Zhang Peng, Sadanandam Abbagani
Format: Article
Language:English
Published: SpringerOpen 2015-12-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
A. tumefaciens
Woodfordia fruticosa
Leaf segments
β-Glucuronidase
hpt
Southern blotting
Online Access:http://www.sciencedirect.com/science/article/pii/S1687157X15000347

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http://www.sciencedirect.com/science/article/pii/S1687157X15000347

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