Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates

Hydrolysis is an often-encountered obstacle in the crystallization of proteins complexed with their substrates. As the duration of the crystallization process, from nucleation to the growth of the crystal to its final size, commonly requires several weeks, non-enzymatic hydrolysis of an “unstab...

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Bibliographic Details
Main Authors: Lutz Schmitt, Erhard Bremer, Sander H. J. Smits, Christine Oswald
Format: Article
Language:English
Published: MDPI AG 2008-07-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/9/7/1131/