Protocol to measure the membrane tension and bending modulus of cells using optical tweezers and scanning electron microscopy

Protocol to measure the membrane tension and bending modulus of cells using optical tweezers and scanning electron microscopy

Summary: The elastic properties of cell membranes, particularly the membrane tension and bending modulus, are known to be key regulators of cellular functions. Here, we present a correlative and integrated tool based on optical tweezers and scanning electron microscopy to accurately determine these...

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Bibliographic Details
Main Authors: Pedro Pompeu, Pedro S. Lourenço, Diney S. Ether, Juliana Soares, Jefte Farias, Gabriela Maciel, Nathan B. Viana, H. Moysés Nussenzveig, Bruno Pontes
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Biophysics
Cell biology
Cell membrane
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302707

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http://www.sciencedirect.com/science/article/pii/S2666166720302707

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