Pulsed Dilution Method for the Recovery of Aggregated Mouse TNF-α
Background: The expression of mouse tumor necrosis factor alpha (TNF-α) in Escherichia coli is a favorable way to get high yield of protein; however, the formation of cytoplasmic inclusion bodies, which is the consequence of insoluble accumulated proteins, is a major obstacle in this system. To over...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Varastegan Institute for Medical Sciences
2017-05-01
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Series: | Reports of Biochemistry and Molecular Biology |
Subjects: | |
Online Access: | http://rbmb.net/article-1-109-en.pdf |