Pulsed Dilution Method for the Recovery of Aggregated Mouse TNF-α

Background: The expression of mouse tumor necrosis factor alpha (TNF-α) in Escherichia coli is a favorable way to get high yield of protein; however, the formation of cytoplasmic inclusion bodies, which is the consequence of insoluble accumulated proteins, is a major obstacle in this system. To over...

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Bibliographic Details
Main Authors: Merat Mahmoodi, Maryam Ghodsi, Malihe Moghadam, Mojtaba Sankian
Format: Article
Language:English
Published: Varastegan Institute for Medical Sciences 2017-05-01
Series:Reports of Biochemistry and Molecular Biology
Subjects:
Online Access:http://rbmb.net/article-1-109-en.pdf