Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells
Background: Rheumatoid arthritis (RA) is a chronic debilitating autoimmune disorder with a high prevalence, especially in industrialized countries. Dysbiosis of the intestinal microbiota has been observed in RA patients. For instance, new-onset untreated RA (NORA) is associated with the underreprese...
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Elsevier
2020-08-01
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daisuke Takahashi Naomi Hoshina Yuma Kabumoto Yuichi Maeda Akari Suzuki Hiyori Tanabe Junya Isobe Takahiro Yamada Kisara Muroi Yuto Yanagisawa Atsuo Nakamura Yumiko Fujimura Aiko Saeki Mizuki Ueda Ryohtaroh Matsumoto Hanako Asaoka Julie M. Clarke Yohsuke Harada Eiji Umemoto Noriko Komatsu Takaharu Okada Hiroshi Takayanagi Kiyoshi Takeda Michio Tomura Koji Hase |
spellingShingle |
Daisuke Takahashi Naomi Hoshina Yuma Kabumoto Yuichi Maeda Akari Suzuki Hiyori Tanabe Junya Isobe Takahiro Yamada Kisara Muroi Yuto Yanagisawa Atsuo Nakamura Yumiko Fujimura Aiko Saeki Mizuki Ueda Ryohtaroh Matsumoto Hanako Asaoka Julie M. Clarke Yohsuke Harada Eiji Umemoto Noriko Komatsu Takaharu Okada Hiroshi Takayanagi Kiyoshi Takeda Michio Tomura Koji Hase Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells EBioMedicine Rheumatoid arthritis Intestinal microbiota Butyrate Follicular regulatory T cells Autoimmunity |
author_facet |
Daisuke Takahashi Naomi Hoshina Yuma Kabumoto Yuichi Maeda Akari Suzuki Hiyori Tanabe Junya Isobe Takahiro Yamada Kisara Muroi Yuto Yanagisawa Atsuo Nakamura Yumiko Fujimura Aiko Saeki Mizuki Ueda Ryohtaroh Matsumoto Hanako Asaoka Julie M. Clarke Yohsuke Harada Eiji Umemoto Noriko Komatsu Takaharu Okada Hiroshi Takayanagi Kiyoshi Takeda Michio Tomura Koji Hase |
author_sort |
Daisuke Takahashi |
title |
Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells |
title_short |
Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells |
title_full |
Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells |
title_fullStr |
Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells |
title_full_unstemmed |
Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells |
title_sort |
microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory t cells |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2020-08-01 |
description |
Background: Rheumatoid arthritis (RA) is a chronic debilitating autoimmune disorder with a high prevalence, especially in industrialized countries. Dysbiosis of the intestinal microbiota has been observed in RA patients. For instance, new-onset untreated RA (NORA) is associated with the underrepresentation of the Clostridium cluster XIVa, including Lachnospiraceae, which are major butyrate producers, although the pathological relevance has remained obscure. Follicular regulatory T (TFR) cells play critical regulatory roles in the pathogenesis of autoimmune diseases, including RA. Reduced number of circulating TFR cells has been associated with the elevation of autoantibodies and disease severity in RA. However, the contribution of commensal microbe-derived butyrate in controlling TFR cell differentiation remains unknown. Methods: We examined the contribution of microbe-derived butyrate in controlling autoimmune arthritis using collagen-induced arthritis (CIA) and SKG arthritis models. We phenotyped autoimmune responses in the gut-associated lymphoid tissues (GALT) in the colon and joint-draining lymph nodes in the CIA model. We developed an in vitro CXCR5+Bcl-6+Foxp3+ TFR (iTFR) cell culture system and examined whether butyrate promotes the differentiation of iTFR cells. Findings: Microbe-derived butyrate suppressed the development of autoimmune arthritis. The immunization of type II collagen (CII) caused hypertrophy of the GALT in the colon by amplifying the GC reaction prior to the onset of the CIA. Butyrate mitigated these pathological events by promoting TFR cell differentiation. Butyrate directly induced the differentiation of functional TFR cells in vitro by enhancing histone acetylation in TFR cell marker genes. This effect was attributed to histone deacetylase (HDAC) inhibition by butyrate, leading to histone hyperacetylation in the promoter region of the TFR-cell marker genes. The adoptive transfer of the butyrate-treated iTFR cells reduced CII-specific autoantibody production and thus ameliorated the symptoms of arthritis. Interpretation: Accordingly, microbiota-derived butyrate serves as an environmental cue to enhance TFR cells, which suppress autoantibody production in the systemic lymphoid tissue, eventually ameliorating RA. Our findings provide mechanistic insights into the link between the gut environment and RA risk. Funding: This work was supported by AMED-Crest (16gm1010004h0101, 17gm1010004h0102, 18gm1010004h0103, and 19gm1010004s0104 to KH), the Japan Society for the Promotion of Science (JP17KT0055, JP16H01369, and JP18H04680 to KH; JP17K15734 to DT), Keio University Special Grant-in-Aid for Innovative Collaborative Research Projects (KH), Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research (DT), the SECOM Science and Technology Foundation (KH), the Cell Science Research Foundation (KH), the Mochida Memorial Foundation for Medical and Pharmaceutical Research (DT), the Suzuken Memorial Foundation (KH and DT), the Takeda Science Foundation (KH and DT), The Science Research Promotion Fund, and The Promotion and Mutual Aid Corporation for Private Schools of Japan (KH). |
topic |
Rheumatoid arthritis Intestinal microbiota Butyrate Follicular regulatory T cells Autoimmunity |
url |
http://www.sciencedirect.com/science/article/pii/S2352396420302887 |
work_keys_str_mv |
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doaj-1f63ab6004a146fa8e8dd2e2492aca392020-11-25T03:20:59ZengElsevierEBioMedicine2352-39642020-08-0158102913Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cellsDaisuke Takahashi0Naomi Hoshina1Yuma Kabumoto2Yuichi Maeda3Akari Suzuki4Hiyori Tanabe5Junya Isobe6Takahiro Yamada7Kisara Muroi8Yuto Yanagisawa9Atsuo Nakamura10Yumiko Fujimura11Aiko Saeki12Mizuki Ueda13Ryohtaroh Matsumoto14Hanako Asaoka15Julie M. Clarke16Yohsuke Harada17Eiji Umemoto18Noriko Komatsu19Takaharu Okada20Hiroshi Takayanagi21Kiyoshi Takeda22Michio Tomura23Koji Hase24Division of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDepartment of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka565-0871, JapanLaboratory for Autoimmune Diseases, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Kanagawa230-0045, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, Japan; Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd., Nishitama, Tokyo190-0182, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanLaboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka584-8540, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, JapanPreventative Health National Research Flagship, CSIRO Food and Nutritional Sciences, Adelaide, South Australia5000, AustraliaLaboratory of Pharmaceutical Immunology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba278-8510, JapanDepartment of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka565-0871, JapanDepartment of Immunology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo113-0033, JapanLaboratory for Tissue Dynamics, RIKEN IMS, Yokohama, Kanagawa230-0045, JapanDepartment of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka565-0871, JapanLaboratory of Pharmaceutical Immunology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba278-8510, JapanLaboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka584-8540, JapanDivision of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo105-8512, Japan; International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo (IMSUT), Minato-ku, Tokyo108-8639, Japan; Corresponding author at: Koji Hase, Division of Biochemistry, Faculty of Pharmacy, Keio University, 1-5-30 Shibakouen, Minatoku, Tokyo 105-8512, JapanBackground: Rheumatoid arthritis (RA) is a chronic debilitating autoimmune disorder with a high prevalence, especially in industrialized countries. Dysbiosis of the intestinal microbiota has been observed in RA patients. For instance, new-onset untreated RA (NORA) is associated with the underrepresentation of the Clostridium cluster XIVa, including Lachnospiraceae, which are major butyrate producers, although the pathological relevance has remained obscure. Follicular regulatory T (TFR) cells play critical regulatory roles in the pathogenesis of autoimmune diseases, including RA. Reduced number of circulating TFR cells has been associated with the elevation of autoantibodies and disease severity in RA. However, the contribution of commensal microbe-derived butyrate in controlling TFR cell differentiation remains unknown. Methods: We examined the contribution of microbe-derived butyrate in controlling autoimmune arthritis using collagen-induced arthritis (CIA) and SKG arthritis models. We phenotyped autoimmune responses in the gut-associated lymphoid tissues (GALT) in the colon and joint-draining lymph nodes in the CIA model. We developed an in vitro CXCR5+Bcl-6+Foxp3+ TFR (iTFR) cell culture system and examined whether butyrate promotes the differentiation of iTFR cells. Findings: Microbe-derived butyrate suppressed the development of autoimmune arthritis. The immunization of type II collagen (CII) caused hypertrophy of the GALT in the colon by amplifying the GC reaction prior to the onset of the CIA. Butyrate mitigated these pathological events by promoting TFR cell differentiation. Butyrate directly induced the differentiation of functional TFR cells in vitro by enhancing histone acetylation in TFR cell marker genes. This effect was attributed to histone deacetylase (HDAC) inhibition by butyrate, leading to histone hyperacetylation in the promoter region of the TFR-cell marker genes. The adoptive transfer of the butyrate-treated iTFR cells reduced CII-specific autoantibody production and thus ameliorated the symptoms of arthritis. Interpretation: Accordingly, microbiota-derived butyrate serves as an environmental cue to enhance TFR cells, which suppress autoantibody production in the systemic lymphoid tissue, eventually ameliorating RA. Our findings provide mechanistic insights into the link between the gut environment and RA risk. Funding: This work was supported by AMED-Crest (16gm1010004h0101, 17gm1010004h0102, 18gm1010004h0103, and 19gm1010004s0104 to KH), the Japan Society for the Promotion of Science (JP17KT0055, JP16H01369, and JP18H04680 to KH; JP17K15734 to DT), Keio University Special Grant-in-Aid for Innovative Collaborative Research Projects (KH), Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research (DT), the SECOM Science and Technology Foundation (KH), the Cell Science Research Foundation (KH), the Mochida Memorial Foundation for Medical and Pharmaceutical Research (DT), the Suzuken Memorial Foundation (KH and DT), the Takeda Science Foundation (KH and DT), The Science Research Promotion Fund, and The Promotion and Mutual Aid Corporation for Private Schools of Japan (KH).http://www.sciencedirect.com/science/article/pii/S2352396420302887Rheumatoid arthritisIntestinal microbiotaButyrateFollicular regulatory T cellsAutoimmunity |