Production and purification of homogenous recombinant human selenoproteins reveals a unique codon skipping event in E. coli and GPX4-specific affinity to bromosulfophthalein

Production and purification of homogenous recombinant human selenoproteins reveals a unique codon skipping event in E. coli and GPX4-specific affinity to bromosulfophthalein

Selenoproteins are translated via animal domain-specific elongation machineries that redefine dedicated UGA opal codons from termination of translation to selenocysteine (Sec) insertion, utilizing specific tRNA species and Sec-specific elongation factors. This has made recombinant production of mamm...

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Bibliographic Details
Main Authors: Qing Cheng, Antonella Roveri, Giorgio Cozza, Luciana Bordin, Isabelle Rohn, Tanja Schwerdtle, Anna Kipp, Fulvio Ursini, Matilde Maiorino, Giovanni Miotto, Elias S.J. Arnér
Format: Article
Language:English
Published: Elsevier 2021-10-01
Series:Redox Biology
Subjects:
Recombinant selenoprotein
Glutathione peroxidase
GPX1
GPX2
GPX4
Frameshift
Online Access:http://www.sciencedirect.com/science/article/pii/S2213231721002299

Internet

http://www.sciencedirect.com/science/article/pii/S2213231721002299

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