Biolistic Transformation of Haematococcus pluvialis With Constructs Based on the Flanking Sequences of Its Endogenous Alpha Tubulin Gene

Biolistic Transformation of Haematococcus pluvialis With Constructs Based on the Flanking Sequences of Its Endogenous Alpha Tubulin Gene

Haematococcus pluvialis has high commercial value, yet it displays low development of genetic transformation systems. In this research, the endogenous 5′ and 3′ flanking sequences of the constitutive alpha tubulin (tub) gene were cloned along with its encoding region in H. pluvialis, in which some p...

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Bibliographic Details
Main Authors: Guanhua Yuan, Xiaoying Xu, Wei Zhang, Wenlei Zhang, Yulin Cui, Song Qin, Tianzhong Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-08-01
Series:Frontiers in Microbiology
Subjects:
alpha tubulin
biolistic transformation
flanking sequence
Haematococcus pluvialis
selection marker
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01749/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.01749/full

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