Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions

The fluorescence in situ hybridization (FISH)-based padlock probe and rolling circle amplification (RCA) method allows for the detection of point mutations. However, it requires multiple reaction steps and solution exchanges, making it costly, labor-intensive, and time-consuming. In this study, we a...

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Bibliographic Details
Main Authors: Yuri Ishigaki, Kae Sato
Format: Article
Language:English
Published: MDPI AG 2018-05-01
Series:Micromachines
Subjects:
Online Access:http://www.mdpi.com/2072-666X/9/6/272