High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing

High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing

The CRISPR/Cas9 prokaryotic adaptive immune system and its swift repurposing for genome editing enables modification of any prespecified genomic sequence with unprecedented accuracy and efficiency, including targeted gene repair. We used the CRISPR/Cas9 system for targeted repair of patient-specific...

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Bibliographic Details
Main Authors: Duran Sürün, Joachim Schwäble, Ana Tomasovic, Roy Ehling, Stefan Stein, Nina Kurrle, Harald von Melchner, Frank Schnütgen
Format: Article
Language:English
Published: Elsevier 2018-03-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
CRISPR/Cas9
non-homologous end joining
NHEJ
in situ gene correction
hematopoietic cells
chronic granulomatous disease
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117302858

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http://www.sciencedirect.com/science/article/pii/S2162253117302858

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