Retention of the Native Epigenome in Purified Mammalian Chromatin.

Retention of the Native Epigenome in Purified Mammalian Chromatin.

A protocol is presented for the isolation of native mammalian chromatin as fibers of 25-250 nucleosomes under conditions that preserve the natural epigenetic signature. The material is composed almost exclusively of histones and DNA and conforms to the structure expected by electron microscopy. All...

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Bibliographic Details
Main Authors: Andreas H Ehrensberger, Don-Marc Franchini, Philip East, Roger George, Nik Matthews, Sarah L Maslen, Jesper Q Svejstrup
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4527833?pdf=render

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http://europepmc.org/articles/PMC4527833?pdf=render

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