Comparison of DNA extraction kits for detection of Burkholderia pseudomallei in spiked human whole blood using real-time PCR.

Burkholderia pseudomallei, the etiologic agent of melioidosis, is endemic in northern Australia and Southeast Asia and can cause severe septicemia that may lead to death in 20% to 50% of cases. Rapid detection of B. pseudomallei infection is crucial for timely treatment of septic patients. This stud...

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Bibliographic Details
Main Authors: Nicole L Podnecky, Mindy G Elrod, Bruce R Newton, Leslie A Dauphin, Jianrong Shi, Sutthinan Chawalchitiporn, Henry C Baggett, Alex R Hoffmaster, Jay E Gee
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3583986?pdf=render