SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species

SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species

Abstract Background The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information. Despite this progress, the availability of high-quality genomic DNA (gDNA) in sufficient concentrations is often a main limitation, espe...

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Bibliographic Details
Main Authors: Santiago Vilanova, David Alonso, Pietro Gramazio, Mariola Plazas, Edgar García-Fortea, Paola Ferrante, Maximilian Schmidt, María José Díez, Björn Usadel, Giovanni Giuliano, Jaime Prohens
Format: Article
Language:English
Published: BMC 2020-08-01
Series:Plant Methods
Subjects:
DNA extraction
CTAB protocol
Silica matrix
Contaminant-free DNA
High-molecular-weight DNA
Next-generation sequencing
Online Access:http://link.springer.com/article/10.1186/s13007-020-00652-y

Internet

http://link.springer.com/article/10.1186/s13007-020-00652-y

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