Murine 3T3-L1 adipocyte cell differentiation model: validated reference genes for qPCR gene expression analysis.

BACKGROUND: Analysis of gene expression at the mRNA level, using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), mandatorily requires reference genes (RGs) as internal controls. However, increasing evidences have shown that RG expression may vary considerably under...

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Bibliographic Details
Main Authors: Tatjana Arsenijevic, Françoise Grégoire, Valérie Delforge, Christine Delporte, Jason Perret
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3358259?pdf=render