A Simple and Efficient CRISPR Technique for Protein Tagging

A Simple and Efficient CRISPR Technique for Protein Tagging

Genetic knock-in using homology-directed repair is an inefficient process, requiring the selection of few modified cells and hindering its application to primary cells. Here, we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in up to 66% of transf...

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Bibliographic Details
Main Authors: Fanning Zeng, Valerie Beck, Sven Schuierer, Isabelle Garnier, Carole Manneville, Claudia Agarinis, Lapo Morelli, Lisa Quinn, Judith Knehr, Guglielmo Roma, Frederic Bassilana, Mark Nash
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Cells
Subjects:
CRISPR knock in
non-homologous end-joining (NHEJ)
protein tagging
Online Access:https://www.mdpi.com/2073-4409/9/12/2618

Internet

https://www.mdpi.com/2073-4409/9/12/2618

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