Developing fluorescence sensor probe to capture activated muscle-specific calpain-3 (CAPN3) in living muscle cells

Developing fluorescence sensor probe to capture activated muscle-specific calpain-3 (CAPN3) in living muscle cells

Calpain-3 (CAPN3) is a muscle-specific type of calpain whose protease activity is triggered by Ca2+. Here, we developed CAPN3 sensor probes (SPs) to detect activated-CAPN3 using a fluorescence/Förster resonance energy transfer (FRET) technique. In our SPs, partial amino acid sequence of calpastatin,...

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Bibliographic Details
Main Authors: Koichi Ojima, Shoji Hata, Fumiko Shinkai-Ouchi, Mika Oe, Susumu Muroya, Hiroyuki Sorimachi, Yasuko Ono
Format: Article
Language:English
Published: The Company of Biologists 2020-09-01
Series:Biology Open
Subjects:
calpain
calpain-3
skeletal muscle
calpainopathy
limb-girdle muscular dystrophy type 2a
proteolysis
Online Access:http://bio.biologists.org/content/9/9/bio048975

Internet

http://bio.biologists.org/content/9/9/bio048975

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