Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)

Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)

Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library preparation strategies relying upon the fusion of locus-specific primers to full-length adapter sequences with a single id...

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Bibliographic Details
Main Authors: Travis C. Glenn, Todd W. Pierson, Natalia J. Bayona-Vásquez, Troy J. Kieran, Sandra L. Hoffberg, Jesse C. Thomas IV, Daniel E. Lefever, John W. Finger, Bei Gao, Xiaoming Bian, Swarnali Louha, Ramya T. Kolli, Kerin E. Bentley, Julie Rushmore, Kelvin Wong, Timothy I. Shaw, Michael J. Rothrock Jr, Anna M. McKee, Tai L. Guo, Rodney Mauricio, Marirosa Molina, Brian S. Cummings, Lawrence H. Lash, Kun Lu, Gregory S. Gilbert, Stephen P. Hubbell, Brant C. Faircloth
Format: Article
Language:English
Published: PeerJ Inc. 2019-10-01
Series:PeerJ
Subjects:
MiSeq
Next generation sequencing
Quadruple indexing
Hierarchical indexing
Multiplexing
Fusion primers
Online Access:https://peerj.com/articles/7786.pdf

Internet

https://peerj.com/articles/7786.pdf

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