Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100

Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100

<p>Abstract</p> <p>Background</p> <p>Controlling and limiting the expression of short hairpin RNA (shRNA) by using constitutive or tissue-specific polymerase II (pol II) expression can be a promising strategy to avoid RNAi toxicity. However, to date detailed studies on...

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Bibliographic Details
Main Authors: Maczuga Piotr, Koornneef Annemart, Borel Florie, Petry Harald, van Deventer Sander, Ritsema Tita, Konstantinova Pavlina
Format: Article
Language:English
Published: BMC 2012-07-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/12/42

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http://www.biomedcentral.com/1472-6750/12/42

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