T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy
Abstract Background The pathological condition of chronic kidney disease may not be adequately recapitulated in immunocompromised mice due to the lack of T-cells, which are important for the development of hypertension and renal injury. We studied the role of the immune system in relation to salt-se...
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doaj-20f70f7705ac4a1a81b11980144e08742020-11-25T02:16:44ZengBMCBMC Nephrology1471-23692017-05-011811510.1186/s12882-017-0555-0T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomyNynke R. Oosterhuis0Diana A. Papazova1Hendrik Gremmels2Jaap A. Joles3Marianne C. Verhaar4Nephrology & Hypertension, University Medical Center UtrechtNephrology & Hypertension, University Medical Center UtrechtNephrology & Hypertension, University Medical Center UtrechtNephrology & Hypertension, University Medical Center UtrechtNephrology & Hypertension, University Medical Center UtrechtAbstract Background The pathological condition of chronic kidney disease may not be adequately recapitulated in immunocompromised mice due to the lack of T-cells, which are important for the development of hypertension and renal injury. We studied the role of the immune system in relation to salt-sensitive hypertension and renal injury in mice with subtotal nephrectomy (SNX). Methods Wild-type immunocompetent (WT) and Foxn1nu/nu athymic immunodeficient (AT) CD-1 mice underwent SNX to induce renal injury after which they received standard chow or a high salt diet (HSD). Four weeks after SNX blood pressure and kidney function parameters were measured. Results HSD increased albumin excretion independent of immune status. Systolic blood pressure increased only in WT mice on HSD, not in AT mice. Uremia and morphological damage after SNX were not affected by either HSD or immune status. Conclusions For the development of hypertension after SNX in CD-1 mice mature T-cells and a high salt diet are required. SNX induced albuminuria was independent of the presence of T-cells.http://link.springer.com/article/10.1186/s12882-017-0555-0AlbuminuriaHigh salt dietHypertensionSubtotal nephrectomyT-cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nynke R. Oosterhuis Diana A. Papazova Hendrik Gremmels Jaap A. Joles Marianne C. Verhaar |
spellingShingle |
Nynke R. Oosterhuis Diana A. Papazova Hendrik Gremmels Jaap A. Joles Marianne C. Verhaar T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy BMC Nephrology Albuminuria High salt diet Hypertension Subtotal nephrectomy T-cells |
author_facet |
Nynke R. Oosterhuis Diana A. Papazova Hendrik Gremmels Jaap A. Joles Marianne C. Verhaar |
author_sort |
Nynke R. Oosterhuis |
title |
T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy |
title_short |
T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy |
title_full |
T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy |
title_fullStr |
T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy |
title_full_unstemmed |
T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy |
title_sort |
t-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy |
publisher |
BMC |
series |
BMC Nephrology |
issn |
1471-2369 |
publishDate |
2017-05-01 |
description |
Abstract Background The pathological condition of chronic kidney disease may not be adequately recapitulated in immunocompromised mice due to the lack of T-cells, which are important for the development of hypertension and renal injury. We studied the role of the immune system in relation to salt-sensitive hypertension and renal injury in mice with subtotal nephrectomy (SNX). Methods Wild-type immunocompetent (WT) and Foxn1nu/nu athymic immunodeficient (AT) CD-1 mice underwent SNX to induce renal injury after which they received standard chow or a high salt diet (HSD). Four weeks after SNX blood pressure and kidney function parameters were measured. Results HSD increased albumin excretion independent of immune status. Systolic blood pressure increased only in WT mice on HSD, not in AT mice. Uremia and morphological damage after SNX were not affected by either HSD or immune status. Conclusions For the development of hypertension after SNX in CD-1 mice mature T-cells and a high salt diet are required. SNX induced albuminuria was independent of the presence of T-cells. |
topic |
Albuminuria High salt diet Hypertension Subtotal nephrectomy T-cells |
url |
http://link.springer.com/article/10.1186/s12882-017-0555-0 |
work_keys_str_mv |
AT nynkeroosterhuis tcellscontributetohypertensionbutnottorenalinjuryinmicewithsubtotalnephrectomy AT dianaapapazova tcellscontributetohypertensionbutnottorenalinjuryinmicewithsubtotalnephrectomy AT hendrikgremmels tcellscontributetohypertensionbutnottorenalinjuryinmicewithsubtotalnephrectomy AT jaapajoles tcellscontributetohypertensionbutnottorenalinjuryinmicewithsubtotalnephrectomy AT mariannecverhaar tcellscontributetohypertensionbutnottorenalinjuryinmicewithsubtotalnephrectomy |
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