Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube

Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube

Abstract Cryopreservation of whole blood is useful for DNA collection, and clinical and basic research. Blood samples in ethylenediaminetetraacetic acid disodium salt (EDTA) tubes stored at − 80 °C are suitable for DNA extraction, but not for high-quality RNA extraction. Herein, a new methodology fo...

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Bibliographic Details
Main Authors: Hirotaka Yamagata, Ayumi Kobayashi, Ryouichi Tsunedomi, Tomoe Seki, Masaaki Kobayashi, Kosuke Hagiwara, Chong Chen, Shusaku Uchida, Go Okada, Manabu Fuchikami, Toshiharu Kamishikiryo, Jun-ichi Iga, Shusuke Numata, Makoto Kinoshita, Takahiro A. Kato, Ryota Hashimoto, Hiroaki Nagano, Yasumasa Okamoto, Shuichi Ueno, Tetsuro Ohmori, Shin Nakagawa
Format: Article
Language:English
Published: Nature Publishing Group 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-96567-2

Internet

https://doi.org/10.1038/s41598-021-96567-2

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