YAP Induces Human Naive Pluripotency
The human naive pluripotent stem cell (PSC) state, corresponding to a pre-implantation stage of development, has been difficult to capture and sustain in vitro. We report that the Hippo pathway effector YAP is nuclearly localized in the inner cell mass of human blastocysts. Overexpression of YAP in...
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doaj-2128dba65f7d4003891ad45c8f7bc6352020-11-25T01:03:33ZengElsevierCell Reports2211-12472016-03-0114102301231210.1016/j.celrep.2016.02.036YAP Induces Human Naive PluripotencyHan Qin0Miroslav Hejna1Yanxia Liu2Michelle Percharde3Mark Wossidlo4Laure Blouin5Jens Durruthy-Durruthy6Priscilla Wong7Zhongxia Qi8Jingwei Yu9Lei S. Qi10Vittorio Sebastiano11Jun S. Song12Miguel Ramalho-Santos13Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USAInstitute for Genomic Biology, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USADepartments of Bioengineering and Chemical and Systems Biology, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USAEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USAEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USAEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USADepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USADepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USADepartments of Bioengineering and Chemical and Systems Biology, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USAInstitute for Genomic Biology, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USAEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USAThe human naive pluripotent stem cell (PSC) state, corresponding to a pre-implantation stage of development, has been difficult to capture and sustain in vitro. We report that the Hippo pathway effector YAP is nuclearly localized in the inner cell mass of human blastocysts. Overexpression of YAP in human embryonic stem cells (ESCs) and induced PSCs (iPSCs) promotes the generation of naive PSCs. Lysophosphatidic acid (LPA) can partially substitute for YAP to generate transgene-free human naive PSCs. YAP- or LPA-induced naive PSCs have a rapid clonal growth rate, a normal karyotype, the ability to form teratomas, transcriptional similarities to human pre-implantation embryos, reduced heterochromatin levels, and other hallmarks of the naive state. YAP/LPA act in part by suppressing differentiation-inducing effects of GSK3 inhibition. CRISPR/Cas9-generated YAP−/− cells have an impaired ability to form colonies in naive but not primed conditions. These results uncover an unexpected role for YAP in the human naive state, with implications for early human embryology.http://www.sciencedirect.com/science/article/pii/S2211124716301395embryonic stem cells (ESCs)induced pluripotent stem cells (iPSCs)pluripotent stem cells (PSCs)naive pluripotencyHippo pathwayYes-associated protein (YAP)lysophosphatidic acid (LPA) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han Qin Miroslav Hejna Yanxia Liu Michelle Percharde Mark Wossidlo Laure Blouin Jens Durruthy-Durruthy Priscilla Wong Zhongxia Qi Jingwei Yu Lei S. Qi Vittorio Sebastiano Jun S. Song Miguel Ramalho-Santos |
spellingShingle |
Han Qin Miroslav Hejna Yanxia Liu Michelle Percharde Mark Wossidlo Laure Blouin Jens Durruthy-Durruthy Priscilla Wong Zhongxia Qi Jingwei Yu Lei S. Qi Vittorio Sebastiano Jun S. Song Miguel Ramalho-Santos YAP Induces Human Naive Pluripotency Cell Reports embryonic stem cells (ESCs) induced pluripotent stem cells (iPSCs) pluripotent stem cells (PSCs) naive pluripotency Hippo pathway Yes-associated protein (YAP) lysophosphatidic acid (LPA) |
author_facet |
Han Qin Miroslav Hejna Yanxia Liu Michelle Percharde Mark Wossidlo Laure Blouin Jens Durruthy-Durruthy Priscilla Wong Zhongxia Qi Jingwei Yu Lei S. Qi Vittorio Sebastiano Jun S. Song Miguel Ramalho-Santos |
author_sort |
Han Qin |
title |
YAP Induces Human Naive Pluripotency |
title_short |
YAP Induces Human Naive Pluripotency |
title_full |
YAP Induces Human Naive Pluripotency |
title_fullStr |
YAP Induces Human Naive Pluripotency |
title_full_unstemmed |
YAP Induces Human Naive Pluripotency |
title_sort |
yap induces human naive pluripotency |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2016-03-01 |
description |
The human naive pluripotent stem cell (PSC) state, corresponding to a pre-implantation stage of development, has been difficult to capture and sustain in vitro. We report that the Hippo pathway effector YAP is nuclearly localized in the inner cell mass of human blastocysts. Overexpression of YAP in human embryonic stem cells (ESCs) and induced PSCs (iPSCs) promotes the generation of naive PSCs. Lysophosphatidic acid (LPA) can partially substitute for YAP to generate transgene-free human naive PSCs. YAP- or LPA-induced naive PSCs have a rapid clonal growth rate, a normal karyotype, the ability to form teratomas, transcriptional similarities to human pre-implantation embryos, reduced heterochromatin levels, and other hallmarks of the naive state. YAP/LPA act in part by suppressing differentiation-inducing effects of GSK3 inhibition. CRISPR/Cas9-generated YAP−/− cells have an impaired ability to form colonies in naive but not primed conditions. These results uncover an unexpected role for YAP in the human naive state, with implications for early human embryology. |
topic |
embryonic stem cells (ESCs) induced pluripotent stem cells (iPSCs) pluripotent stem cells (PSCs) naive pluripotency Hippo pathway Yes-associated protein (YAP) lysophosphatidic acid (LPA) |
url |
http://www.sciencedirect.com/science/article/pii/S2211124716301395 |
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