Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining

<p>Abstract</p> <p>Background</p> <p>GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybridization (FISH). Direct...

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Bibliographic Details
Main Authors: Rohr Karl, Eils Roland, Otten Simone, Knerr Boris, Nagel Jens, Kim Il-Han, Dietzel Steffen
Format: Article
Language:English
Published: BMC 2007-12-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/7/92