Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil
Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 past...
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Sociedade Brasileira de Microbiologia
2014-06-01
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doaj-21efe3ae599e4b468076fb9003d078d42020-11-25T01:57:44ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052014-06-0145270771110.1590/S1517-83822014000200046S1517-83822014000200046Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, BrazilSônia Aparecida Sgarioni0Rosario Dominguez Crespo Hirata1Mario Hiroyuki Hirata2Clarice Queico Fujimura Leite3KarinaAndrade de Prince4Sergio Roberto de Andrade Leite5Dirceu Vedovello Filho6Vera Lucia Dias Siqueira7Katiany Rizzieri Caleffi-Ferracioli8Rosilene Fressatti Cardoso9Universidade Estadual de MaringáUniversidade de São PauloUniversidade de São PauloUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaSecretaria de Saúde do Estado do ParanáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáMilk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046&lng=en&tlng=ennon-tuberculous mycobacteriamilkMycobacteriumPCR-PRAmycolic acids analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sônia Aparecida Sgarioni Rosario Dominguez Crespo Hirata Mario Hiroyuki Hirata Clarice Queico Fujimura Leite KarinaAndrade de Prince Sergio Roberto de Andrade Leite Dirceu Vedovello Filho Vera Lucia Dias Siqueira Katiany Rizzieri Caleffi-Ferracioli Rosilene Fressatti Cardoso |
spellingShingle |
Sônia Aparecida Sgarioni Rosario Dominguez Crespo Hirata Mario Hiroyuki Hirata Clarice Queico Fujimura Leite KarinaAndrade de Prince Sergio Roberto de Andrade Leite Dirceu Vedovello Filho Vera Lucia Dias Siqueira Katiany Rizzieri Caleffi-Ferracioli Rosilene Fressatti Cardoso Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil Brazilian Journal of Microbiology non-tuberculous mycobacteria milk Mycobacterium PCR-PRA mycolic acids analysis |
author_facet |
Sônia Aparecida Sgarioni Rosario Dominguez Crespo Hirata Mario Hiroyuki Hirata Clarice Queico Fujimura Leite KarinaAndrade de Prince Sergio Roberto de Andrade Leite Dirceu Vedovello Filho Vera Lucia Dias Siqueira Katiany Rizzieri Caleffi-Ferracioli Rosilene Fressatti Cardoso |
author_sort |
Sônia Aparecida Sgarioni |
title |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_short |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_full |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_fullStr |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_full_unstemmed |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_sort |
occurrence of mycobacterium bovis and non-tuberculous mycobacteria (ntm) in raw and pasteurized milk in the northwestern region of paraná, brazil |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2014-06-01 |
description |
Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil. |
topic |
non-tuberculous mycobacteria milk Mycobacterium PCR-PRA mycolic acids analysis |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046&lng=en&tlng=en |
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