Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker

Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker

CD63, a member of the tetraspanin superfamily, is used as a marker of late endosomes and lysosome-related organelles, as well as a marker of exosomes. Here, we selected rare isotype variants of TS63 by sorting hybridoma cells on the basis of their high expression of surface immunoglobulins of the Ig...

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Bibliographic Details
Main Authors: Stéphanie Charrin, Roberta Palmulli, Martine Billard, Denis Clay, Claude Boucheix, Guillaume Van Niel, Eric Rubinstein
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Antibodies
Subjects:
CD63
exosomes
late endosomes
isotype switch
Online Access:https://www.mdpi.com/2073-4468/9/3/29

Internet

https://www.mdpi.com/2073-4468/9/3/29

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