Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showe...

Full description

Bibliographic Details
Main Authors: Galina S. Zamay, Olga S. Kolovskaya, Tatiana I. Ivanchenko, Tatiana N. Zamay, Dmitry V. Veprintsev, Valentina L. Grigorieva, Irina I. Garanzha, Alexey V. Krat, Yury E. Glazyrin, Ana Gargaun, Ivan N. Lapin, Valery A. Svetlichnyi, Maxim V. Berezovski, Anna S. Kichkailo
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:Cancers
Subjects:
aptamers
SELEX (Systematic evolution of ligands by exponential enrichment)
EpCAM
non-small-cell lung cancer
circulating tumor cells
blood
Online Access:http://www.mdpi.com/2072-6694/11/3/351

Internet

http://www.mdpi.com/2072-6694/11/3/351

Similar Items