Improved mycelia and polysaccharide production of Grifola frondosa by controlling morphology with microparticle Talc

• Main Authors: Ting-Lei Tao, Feng-Jie Cui, Xiao-Xiao Chen, Wen-Jing Sun, Da-Ming Huang, Jinsong Zhang, Yan Yang, Di Wu, Wei-Min Liu
• Format: Article
• Language: English
• Published: BMC 2018-01-01
• Series: Microbial Cell Factories
• Subjects: Grifola frondosa; Microparticle Talc; Mycelial morphology; Polysaccharide biosynthesis; Enzyme activities
• Online Access: http://link.springer.com/article/10.1186/s12934-017-0850-2

Abstract Background Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation. Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production. Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermentation performance, monosaccharide compositions of polysaccharides and enzymes activities associated with polysaccharide synthesis in G. frondosa was well investigated to find a clue of the relationship between polysaccharide biosynthesis and morphological changes. Results Addition of Talc decreased the diameter of the pellets and increased the percentage of S-fraction mycelia. Talc gave the maximum mycelial biomass of 19.25 g/L and exo-polysaccharide of 3.12 g/L at 6.0 g/L of Talc, and mycelial polysaccharide of 0.24 g/g at 3.0 g/L of Talc. Talc altered the monosaccharide compositions/percentages in G. frondosa mycelial polysaccharide with highest mannose percentage of 62.76 % and lowest glucose percentage of 15.22 % followed with the corresponding changes of polysaccharide-synthesis associated enzymes including lowest UDP-glucose pyrophosphorylase (UGP) activity of 91.18 mU/mg and highest UDP-glucose dehydrogenase (UGDG) and GDP-mannose pyrophosphorylase (GMPPB) activities of 81.45 mU/mg and 93.15 mU/mg. Conclusion Our findings revealed that the presence of Talc significantly changed the polysaccharide production and sugar compositions/percentages in mycelial and exo-polysaccharides by affecting mycelial morphology and polysaccharide-biosynthesis related enzymes activities of G. frondosa.