DCM-related tropomyosin mutants E40K/E54K over-inhibit the actomyosin interaction and lead to a decrease in the number of cycling cross-bridges.

Two DCM mutants (E40K and E54K) of tropomyosin (Tm) were examined using the thin-filament extraction/reconstitu-tion technique. The effects of the Ca²⁺, ATP, phos-phate (Pi), and ADP concentrations on isometric tension and its transients were studied at 25°C, and the results were com-pared to those...

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Bibliographic Details
Main Authors: Fan Bai, Heather L Groth, Masataka Kawai
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3471818?pdf=render