Comparing two protocols of DNA extraction of Trypanosoma cruzi cultured in axenic medium

Objectives. To compare two extraction protocols of Trypanosoma cruzi DNA for use in DNA amplification of kinetoplast minicircles (kDNA) through the technique of Polymerase Chain Reaction (PCR). Materials and methods. Epimastigotes of T. cruzi were cultured in axenic conditions and masses from 1.5 to...

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Bibliographic Details
Main Authors: Mariela López, María G. Rivera, Mercedes Viettri, María Lares, Antonio Morocoima, Leidi Herrera, Elizabeth Ferrer
Format: Article
Language:Spanish
Published: Instituto Nacional de Salud 2014-07-01
Series:Revista Peruana de Medicina Experimental y Salud Pública
Subjects:
adn
Online Access:https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/38