Comparing two protocols of DNA extraction of Trypanosoma cruzi cultured in axenic medium
Objectives. To compare two extraction protocols of Trypanosoma cruzi DNA for use in DNA amplification of kinetoplast minicircles (kDNA) through the technique of Polymerase Chain Reaction (PCR). Materials and methods. Epimastigotes of T. cruzi were cultured in axenic conditions and masses from 1.5 to...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | Spanish |
Published: |
Instituto Nacional de Salud
2014-07-01
|
Series: | Revista Peruana de Medicina Experimental y Salud Pública |
Subjects: | |
Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/38 |