Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography
A simple and reliable method for the determination of the concentration and function of alpha-2-macroglobulin (α2M) by zymography was developed. The method is based on the covalent binding of α2M and trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the electrophoret...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Serbian Chemical Society
2019-01-01
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Series: | Journal of the Serbian Chemical Society |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391900051G.pdf |