Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes.
Formation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes. Vertebrate oocytes present a RanGTP gradient centred on chromatin at all stages of meiotic maturation. However, this...
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doaj-241fdf63521c49f29d220e7a5832feaa2020-11-25T01:46:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01310e333810.1371/journal.pone.0003338Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes.Stéphane BrunetJulien DumontKaren W LeeKazuhisa KinoshitaPascale HikalOliver J GrussBernard MaroMarie-Hélène VerlhacFormation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes. Vertebrate oocytes present a RanGTP gradient centred on chromatin at all stages of meiotic maturation. However, this gradient is dispensable for assembly of the first meiotic spindle. To understand this meiosis I peculiarity, we studied TPX2, a Ran target, in mouse oocytes. Strikingly, TPX2 activity is controlled at the protein level through its accumulation from meiosis I to II. By RNAi depletion and live imaging, we show that TPX2 is required for spindle assembly via two distinct functions. It controls microtubule assembly and spindle pole integrity via the phosphorylation of TACC3, a regulator of MTOCs activity. We show that meiotic spindle formation in vivo depends on the regulation of at least a target of Ran, TPX2, rather than on the regulation of the RanGTP gradient itself.http://europepmc.org/articles/PMC2556383?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stéphane Brunet Julien Dumont Karen W Lee Kazuhisa Kinoshita Pascale Hikal Oliver J Gruss Bernard Maro Marie-Hélène Verlhac |
spellingShingle |
Stéphane Brunet Julien Dumont Karen W Lee Kazuhisa Kinoshita Pascale Hikal Oliver J Gruss Bernard Maro Marie-Hélène Verlhac Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. PLoS ONE |
author_facet |
Stéphane Brunet Julien Dumont Karen W Lee Kazuhisa Kinoshita Pascale Hikal Oliver J Gruss Bernard Maro Marie-Hélène Verlhac |
author_sort |
Stéphane Brunet |
title |
Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. |
title_short |
Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. |
title_full |
Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. |
title_fullStr |
Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. |
title_full_unstemmed |
Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. |
title_sort |
meiotic regulation of tpx2 protein levels governs cell cycle progression in mouse oocytes. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-01-01 |
description |
Formation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes. Vertebrate oocytes present a RanGTP gradient centred on chromatin at all stages of meiotic maturation. However, this gradient is dispensable for assembly of the first meiotic spindle. To understand this meiosis I peculiarity, we studied TPX2, a Ran target, in mouse oocytes. Strikingly, TPX2 activity is controlled at the protein level through its accumulation from meiosis I to II. By RNAi depletion and live imaging, we show that TPX2 is required for spindle assembly via two distinct functions. It controls microtubule assembly and spindle pole integrity via the phosphorylation of TACC3, a regulator of MTOCs activity. We show that meiotic spindle formation in vivo depends on the regulation of at least a target of Ran, TPX2, rather than on the regulation of the RanGTP gradient itself. |
url |
http://europepmc.org/articles/PMC2556383?pdf=render |
work_keys_str_mv |
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