Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling

Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling

Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect...

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Bibliographic Details
Main Authors: Giuseppa De Luca, Barbara Cardinali, Lucia Del Mastro, Sonia Lastraioli, Franca Carli, Manlio Ferrarini, George A. Calin, Anna Garuti, Carlotta Mazzitelli, Simona Zupo, Mariella Dono
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:International Journal of Molecular Sciences
Subjects:
Circulating Tumor Cells
breast cancer
liquid biopsy
Next-Generation Sequencing
molecular tagging
Whole Genome Amplification-free
Online Access:https://www.mdpi.com/1422-0067/21/12/4364

Internet

https://www.mdpi.com/1422-0067/21/12/4364

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