The Application of Transient-State Kinetic Isotope Effects to the Resolution of Mechanisms of Enzyme-Catalyzed Reactions
Much of our understanding of the mechanisms of enzyme-catalyzed reactions is based on steady-state kinetic studies. Experimentally, this approach depends solely on the measurement of rates of free product appearance (d[P]/dt), a mechanistically and mathematically complex entity. Despite the ambiguit...
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Format: | Article |
Language: | English |
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MDPI AG
2013-07-01
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Series: | Molecules |
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Online Access: | http://www.mdpi.com/1420-3049/18/7/8230 |