The Application of Transient-State Kinetic Isotope Effects to the Resolution of Mechanisms of Enzyme-Catalyzed Reactions

The Application of Transient-State Kinetic Isotope Effects to the Resolution of Mechanisms of Enzyme-Catalyzed Reactions

Much of our understanding of the mechanisms of enzyme-catalyzed reactions is based on steady-state kinetic studies. Experimentally, this approach depends solely on the measurement of rates of free product appearance (d[P]/dt), a mechanistically and mathematically complex entity. Despite the ambiguit...

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Bibliographic Details
Main Author: Harvey F. Fisher
Format: Article
Language:English
Published: MDPI AG 2013-07-01
Series:Molecules
Subjects:
enzyme mechanism
isotope effects
isotope sensitive step
stopped-flow
quench flow
Online Access:http://www.mdpi.com/1420-3049/18/7/8230

Internet

http://www.mdpi.com/1420-3049/18/7/8230

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