Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells
The electronic cigarettes mimic combustible cigarettes through a heating technology that vaporizes a refill liquid consisting of solvents, flavors, and nicotine. E-cigarettes are sometimes still used as a support for smoking cessation, even if in 2019 an acute lung injury outbreak occurred in the US...
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doaj-24abfb4683e242baaf5265fa66bd397d2021-03-05T04:28:34ZengElsevierToxicology Reports2214-75002021-01-018456462Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cellsSara De Martin0Daniela Gabbia1Sara Bogialli2Franco Biasioli3Andrea Boschetti4Ronald Gstir5Daniela Rainer6Luca Cappellin7Department of Pharmaceutical and Pharmacological Sciences, Università degli Studi di Padova, Padova, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, Università degli Studi di Padova, Padova, ItalyDepartment of Chemical Sciences, Università degli Studi di Padova, Padova, ItalyResearch and Innovation Centre, Fondazione Edmund Mach, S. Michele a/A, ItalyCNR-IMEM, Sezione di Trento, 38050, Povo, Trento, ItalyAustrian Drug Screening Institute GmbH, Innsbruck, AustriaAustrian Drug Screening Institute GmbH, Innsbruck, AustriaDepartment of Chemical Sciences, Università degli Studi di Padova, Padova, Italy; Research and Innovation Centre, Fondazione Edmund Mach, S. Michele a/A, Italy; Corresponding author at: Department of Chemical Sciences, Università degli Studi di Padova, Padova, Italy.The electronic cigarettes mimic combustible cigarettes through a heating technology that vaporizes a refill liquid consisting of solvents, flavors, and nicotine. E-cigarettes are sometimes still used as a support for smoking cessation, even if in 2019 an acute lung injury outbreak occurred in the USA, affecting mainly adolescents and young adults, and was correlated to eCigs. Therefore, due to the lack of a definite knowledge about the mechanism(s) of refill liquid toxicity and considering that previous investigations gave controversial results, the aim of the present study was the cytotoxicity assessment of different refill liquids on human endothelial cells, evaluated by means of two different in vitro approaches, i.e. the resazurin and the LDH release assays. Our results clearly demonstrated that different refill liquids (6 samples) display different levels of cytotoxicity in our cellular model, although their cytotoxicity was always lower than that observed for the condensate obtained from traditional cigarettes (3 samples). These results suggest that accurate evaluations should be provided for refill liquids, in particular to correlate their toxicity to their chemical composition, with the final aim of obtaining useful information for the agencies involved in the regulation of their components.http://www.sciencedirect.com/science/article/pii/S221475002100038XElectronic cigarettesRefill liquidsCytotoxicityEVALI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sara De Martin Daniela Gabbia Sara Bogialli Franco Biasioli Andrea Boschetti Ronald Gstir Daniela Rainer Luca Cappellin |
spellingShingle |
Sara De Martin Daniela Gabbia Sara Bogialli Franco Biasioli Andrea Boschetti Ronald Gstir Daniela Rainer Luca Cappellin Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells Toxicology Reports Electronic cigarettes Refill liquids Cytotoxicity EVALI |
author_facet |
Sara De Martin Daniela Gabbia Sara Bogialli Franco Biasioli Andrea Boschetti Ronald Gstir Daniela Rainer Luca Cappellin |
author_sort |
Sara De Martin |
title |
Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells |
title_short |
Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells |
title_full |
Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells |
title_fullStr |
Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells |
title_full_unstemmed |
Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells |
title_sort |
refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells |
publisher |
Elsevier |
series |
Toxicology Reports |
issn |
2214-7500 |
publishDate |
2021-01-01 |
description |
The electronic cigarettes mimic combustible cigarettes through a heating technology that vaporizes a refill liquid consisting of solvents, flavors, and nicotine. E-cigarettes are sometimes still used as a support for smoking cessation, even if in 2019 an acute lung injury outbreak occurred in the USA, affecting mainly adolescents and young adults, and was correlated to eCigs. Therefore, due to the lack of a definite knowledge about the mechanism(s) of refill liquid toxicity and considering that previous investigations gave controversial results, the aim of the present study was the cytotoxicity assessment of different refill liquids on human endothelial cells, evaluated by means of two different in vitro approaches, i.e. the resazurin and the LDH release assays. Our results clearly demonstrated that different refill liquids (6 samples) display different levels of cytotoxicity in our cellular model, although their cytotoxicity was always lower than that observed for the condensate obtained from traditional cigarettes (3 samples). These results suggest that accurate evaluations should be provided for refill liquids, in particular to correlate their toxicity to their chemical composition, with the final aim of obtaining useful information for the agencies involved in the regulation of their components. |
topic |
Electronic cigarettes Refill liquids Cytotoxicity EVALI |
url |
http://www.sciencedirect.com/science/article/pii/S221475002100038X |
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