Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

Abstract Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive option. In the present study, various ch...

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Bibliographic Details
Main Authors: Natsuko Miura, Kana Miyamoto, Yuta Ohtani, Kenshi Yaginuma, Shunsuke Aburaya, Yoshinori Kitagawa, Wataru Aoki, Mitsuyoshi Ueda
Format: Article
Language:English
Published: SpringerOpen 2019-07-01
Series:AMB Express
Subjects:
CDR swapping
CD4 nanobody
GFP nanobody
Relative binding ability
Pichia pastoris
Online Access:http://link.springer.com/article/10.1186/s13568-019-0833-2

Internet

http://link.springer.com/article/10.1186/s13568-019-0833-2

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