High-throughput isolation of giant viruses in liquid medium using automated flow cytometry and fluorescence staining.

High-throughput isolation of giant viruses in liquid medium using automated flow cytometry and fluorescence staining.

The isolation of giant viruses using amoeba co-culture is tedious and fastidious. Recently, the procedure was successfully associated with a method that detects amoebal lysis on agar plates. However, the procedure remains time-consuming and is limited to protozoa growing on agar. We present here adv...

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Bibliographic Details
Main Authors: Jacques Yaacoub Bou Khalil, stephane eRobert, Dorine gaëlle Reteno, Julien eAndreani, Didier eRaoult, Bernard eLa Scola
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-01-01
Series:Frontiers in Microbiology
Subjects:
Flow Cytometry
protozoa
high-throughput
giant viruses
automated system
Gating strategy
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00026/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00026/full

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