Comparative study of protease hydrolysis reaction demonstrating Normalized Peptide Bond Cleavage Frequency and Protease Substrate Broadness Index.

Two commercial proteases (subtilisin-typed FNA from Bacillus amyloliquefaciens, and chymotrypsin-like NPP from Nocardiopsis prasina), porcine pepsin, porcine pancreatin having protease activity and their combinations were studied in vitro by LC-MS for their ability to digest soy protein isolate (SPI...

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Bibliographic Details
Main Authors: Shukun Yu, Janne Bech Thoegersen, Karsten Mathias Kragh
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0239080