Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has facilitated dramatic progress in the field of genome engineering. Whilst microinjection of the Cas9 protein and a single guide RNA (sgRNA) into mouse zygotes is a widespread method for producing genetical...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-50900-y |