PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts

PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology allows the modification of DNA sequences in vivo at the location of interest. Although CRISPR-Cas9 can produce genomic changes that do not require DNA vector carriers, the use of transgenesis for the stable integratio...

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Bibliographic Details
Main Authors: Rodrigo Ribeiro Arnt Sant’Ana, Clarissa Alves Caprestano, Rubens Onofre Nodari, Sarah Zanon Agapito-Tenfen
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Genes
Subjects:
gene editing
mutagenesis
genetically modified
genetically modified organism (GMO)
crop breeding
ribonucleoprotein complex (RNP)
Online Access:https://www.mdpi.com/2073-4425/11/9/1029

Internet

https://www.mdpi.com/2073-4425/11/9/1029

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