Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Abstract Background Careful consideration of experimental artefacts is required in order to successfully apply high-throughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, quality control and “denoising” approaches for sequencing low biomas...

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Bibliographic Details
Main Authors: Shantelle Claassen-Weitz, Sugnet Gardner-Lubbe, Kilaza S. Mwaikono, Elloise du Toit, Heather J. Zar, Mark P. Nicol
Format: Article
Language:English
Published: BMC 2020-05-01
Series:BMC Microbiology
Subjects:
16S rRNA gene
Bacteriome
Contamination
High-throughput sequencing
Low biomass
Mock controls
Online Access:http://link.springer.com/article/10.1186/s12866-020-01795-7

Internet

http://link.springer.com/article/10.1186/s12866-020-01795-7

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