Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Abstract Background Careful consideration of experimental artefacts is required in order to successfully apply high-throughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, quality control and “denoising” approaches for sequencing low biomas...

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Bibliographic Details
Main Authors: Shantelle Claassen-Weitz, Sugnet Gardner-Lubbe, Kilaza S. Mwaikono, Elloise du Toit, Heather J. Zar, Mark P. Nicol
Format: Article
Published: BMC 2020-05-01
Series:BMC Microbiology
Online Access:http://link.springer.com/article/10.1186/s12866-020-01795-7